![]() ![]() Furthermore, filamin-B variants lacking the H1 region induce the formation of thinner myotubes than those in cells containing variants with this region. When expressed in C2C12 cells, filamin-B var-1(ΔH1) accelerates their differentiation into myotubes. Interestingly, the simultaneous deletion of the H1 region is required for the localization of filamin-B at the tips of actin stress fibers. The mere presence of the high-affinity binding site for β1A is not sufficient for targeting the filamin-B var-1 construct to focal contacts. We show that both filamin-A var-1 and filamin-B var-1 bind more strongly than their wild-type isoforms to different integrin β subunits. Furthermore, alternative splice variants of filamin-B and filamin-C, from which the flexible hinge-1 region is deleted (ΔH1), were induced during in vitro differentiation of C2C12 mouse myoblasts. RT-PCR analysis showed weak, but wide, expression of filamin-B var-1 and a similar splice variant of filamin-A (filamin-A var-1) in human tissues. Using the yeast two-hybrid system, we isolated a novel splice variant (filamin-B var-1) of the filamentous actin cross-linking protein, filamin-B, that interacts with the cytoplasmic domain of the integrin β1A and β1D subunits. Integrins connect the extracellular matrix with the cell interior, and transduce signals through interactions of their cytoplasmic tails with cytoskeletal and signaling proteins. ![]()
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